Bunnik Lab

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Handbook of Operations

Environmental Health & Safety

  Medium for Plasmodium falciparum

  Culturing 293T cells

  Culturing 3T3 cells

  Culturing THP-1 cells

  Memory B-cell isolation

  Transfection of 293T cells

  Thawing cell lines

  Human IgG ELISA

  Mycoplasma test

  cDNA synthesis using RNA and qScript XLT
CHEMICALS - SAFETY INFORMATION



Beta-mercaptoethanol or BME or 2-mercaptoethanol
- Health hazard 1 (slightly hazardous)


Bleach or hypochloride (8.25%)
- Health hazard 2 (moderately hazardous)


Giemsa stain solution
- Health hazard 2 (moderately hazardous)


Hydrogen chloride (HCl) 1M
- Health hazard 3 (seriously hazardous)


IgG elution buffer (pH 2.0)
- Health hazard 2 (moderately hazardous)


Kanamycin sulfate powder
- Health hazard 2 (moderately hazardous)
| SOP

Methanol
- Health hazard 1 (slightly hazardous)


Mitomycin C
- Health hazard 4 (severely hazardous)


SimplyBlue safe stain
- Health hazard 0 (not hazardous)


Sodium hydroxide (NaOH) solution (1M)
- Health hazard 3 (seriously hazardous)


Sulfuric acid or H2SO4
- Health hazard 3 (seriously hazardous)


TMB blotting solution
- Health hazard 0 (not hazardous)


TMB (for ELISA)
- Health hazard 1 (slightly hazardous)


TRI-reagent
- Health hazard 3 (seriously hazardous)


Triton X-100
- Health hazard 4, oral (severely hazardous), health hazard 1, eyes (slightly hazardous)


Trypan blue
- Health hazard 1 (slightly hazardous)
| SOP
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