Bunnik lab
In the Bunnik lab, we study malaria and B cells.
We are currently looking for people to join the lab. Motivated students, postdocs and technicians are encouraged to contact Dr. Bunnik.
Antibody C7 binds CIDRα1 with its heavy and light chain at the site where CIDRα1 would otherwise bind human endothelial receptor EPCR (shown as EB & EBS).
The Bunnik lab identified antibodies that bind and inhibit most PfEMP1 CIDRα1 variants, thereby preventing infected erythrocytes from binding to human endothelial receptor EPCR. Parasite binding to EPCR is believed to be the major pathogenic pathway in severe malaria.
B cells were isolated from blood donated by people in Uganda, Africa and cultured in the lab. After screening culture supernatant for IgG production, antibody variable regions were amplified and cloned into expression constructs, which allowed for unlimited production of these CIDRα1-specific antibodies.
In collaboration with several other labs around the globe, we identified the key residues of the broadly inhibitory antibodies interacting with Plasmodium falciparum protein PfEMP1 CIDRα1.
The results of this study will be published in Nature later this year.
San Antonio, TX | 2024